Microbiology Review Questions
Week10ReviewSheet I
Exercise1-Disinfectants
1. Whatdoesbactericidalmean?Bacteriostatic?Virucidal?Fungistatic?
2. Whyarecontrolculturesnecessaryinevaluatingdisinfectants?
3. Whatfactorscaninfluencetheactivityofadisinfectant?
4. Whydomicroorganismsdifferintheirresponsetodisinfectants?
5. Whatmicroorganismsaremostsusceptibleto disinfectants?
EXERCISE2-AntimicrobialAgentSusceptibilityTestingand Resistance
1. Whatismeantbyantimicrobialresistance?Susceptibility?
2. Whyarepureculturesusedforantimicrobialsusceptibilitytesting?
3. Woulditbeacceptabletouseamixedcultureforthistest?Why?
4. Listthreefactorsthatcaninfluencetheaccuracyofthetest.
5. Whenperformingabrothdilutiontest,whyis itnecessaryto includea growthcontroltube?
Asterilitycontroltube?
EXERCISE3-TheEnterobacteriaceae(EntericBacilli)
1. Whatdoestheterm IMViCmean?
2. Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?
3. WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other
Enterobacteriaceae?
4. HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?
5. Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?
EXERCISE4–Streptococci,Pneumococci,and Enterococci
1. Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby
Gramstain.
2. Whattypeof hemolysisis producedbyS. pneumoniae?
3. HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?
4. Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci.
5. Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?
EXERCISE5-UrineCultureTechniques
1. Whatis bacteriuria?Whenis itsignificant?
2. Howdomicroorganismsentertheurinarytract?
3. Whyisasepticurinecollectionimportantwhenculturesareordered?
4. Listfivebacteriathatcancauseurinarytractinfection.
5. Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?
Week10ReviewSheet II
EXERCISE1-SerodiagnosisofInfectiousDisease
1. Defineserumtiter.
2. Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?
3. Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?
4. Ina pairedserumsample,whattestresultsindicaterecentinfection?
5. Whatis ahumoralantibody?
EXERCISE2-CulturingMicroorganismsfromtheEnvironment
1. Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?
2. Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?
3. Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?
4. Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?
5. Whenandwhyis handwashingimportantinpatientcare?
6. Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?
EXERCISE3-Staphylococci
1. Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby
Gramstain.
2. Whatarethetwotypesofstaphylococcalcoagulase?
3. Whatis proteinA?Describeonemethodofdetectingit.
4. Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?
5. Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?
__________________________________________________________________________Must have APA citation and include questions and answers.
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